The secondary structures are essentially orientated parallel to the air/water interface. This transition could be observed in the compression isotherm as well as during the adsorption at the air/water interface from the subphase as a function of time. For the peptide films the shape and position of the amide I and amide II bands indicate that the KLAL adopts at large areas per molecule an α-helical secondary structure, whereas at higher surface pressures or smaller areas it converts into a β-sheet structure. The linear sequence KLAL (KLALKLALKALKAALKLA-NH 2) and its corresponding d,l-isomers k 9a 10-KLAL (KLALKLALkaLKAALKLA-NH 2) and l 11k 12-KLAL (KLALKLALKAlkAALKLA-NH 2) are model compounds for potentially amphipathic α-helical peptides which are able to bind to membranes and to increase the membrane permeability in a structure- and target-dependent manner ( Dathe and Wieprecht, 1999) We first studied the secondary structure of KLAL and its analogs bound to the air/water using infrared reflection absorption spectroscopy.
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